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MUNRO Anaerobic Chambers

MUNRO Anaerobic Chambers

Munro’s anaerobic chambers provide a cost-effective means to work with samples in a tightly controlled and sustainable environment.  Sympathetic design features permit fast and efficient working whilst a completely sealed and positively pressurised workstation ensures samples are not exposed to oxygen.

A range of designs are available to cater to the individual needs of clients, including quick access ports, additional environmental controls, a wide range of internal configurations and multiple handling apertures.

About Anaerobic Chambers

The anaerobic chamber provides an oxygen free (anaerobic) environment by forming an airtight vessel from which the existing atmosphere can be purged.  First, samples to be worked on are placed in the workstation.  Next the airtight chamber(s) are flushed with gas.  Depending on the setup cheaper nitrogen can be used to initially purge the system, or the oxygen can be pushed out by the desired mix of gases.  With the chamber now holding the desired mix of gases, the atmosphere is maintained by a series of airlocks and gloved handling ports, coupled with a constant positive pressure to ensure any breaches result in a flow of gas out of the chamber not a flow of contaminating gas in.  A reactive catalyst sits within the chamber, pulling any remaining oxygen from the atmosphere.  Samples can be transferred to or from the chamber by the airlock or a quick release, and the workstation provides ample space to store and process samples within the controlled atmosphere.

Gases in use will vary depending on application and location.  Whilst here in the UK we have access to a wide range of gases, it is not always so easy to source these.  Munro’s cambers are designed to work with nitrogen, carbon dioxide and hydrogen, in various mixtures as available and/or required.  Nitrogen is an inexpensive and safe means to fill the cabinet and displace oxygen.  Carbon dioxide provides a stimulant for the growth of bacteria.  Hydrogen is used to further reduce the levels of oxygen in the chamber.

MUNRO Anaerobic Chambers

Uses of Anaerobic Chambers

Anaerobic cabinets are used for a wide range of scientific and industrial purposes, including but not limited to clinical microbiology, biochemistry, microbiome study, dental study, cell culture and biofuels.  With the comfortable gloved access, and handy airlock ports, any work that could be conducted on a bench can be conducted with similar ease in a controlled atmosphere.

MUNRO Anaerobic Chambers

Designed and built by Munro in our UK manufacturing facility, our anaerobic chambers benefit from a long history of expertise in the production of precision equipment.  The chambers feature everything that is required and nothing that isn’t, ensuring excellent value for money and a cost-effective purchase.  As with all of our equipment, we provide fast, stress-free, worldwide shipping.

Our anaerobic video

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Types of Anemometers

Types of Anemometers

 

An anemometer, or wind anemometer, is a device that monitors air velocity. Historically, the early anemometers were used to estimate the direction and speed of the wind. As technology improves, anemometers can now offer a wide range of very precise information. There are a variety of applications for anemometers in both residential and commercial environments. They can measure Inconsistent sources of wind velocity data, high-speed readings, etc.

Handheld Anemometers

These anemometers can measure wind speeds to 30 m/s. The speed is around 108 miles an hour with approximately 2% accuracy. The Handheld anemometer is powered by a single AA battery. If you’re planning to go camping, hiking, sailing, or hunting and need to know the direction or speed of the wind, this is the ideal tool for you.

Handheld Anemometers

Hot Wire Anemometer

The wind speed and direction may be determined with this instrument. Temperature differences between hot and cold airflow are used to determine wind speed in hot-wire anemometers. Being a heat transducer, a hot-wire anemometer monitors the instantaneous flow rate. The use of a hot-wire anemometer allows the flow velocity to be computed from electric voltage readings at any given time.

Vane Anemometers:

The wind speed is measured when the turbine of the vane anemometer rotates, and the propeller’s magnet passes through the reed switch. The value in a certain amount of time of the number of times the magnetized blade passes over the reed switch is counted.

Vane Anemometer

Cup Anemometer:

In this type of anemometer, a vertical rod is present on which cups, three in number, are mounted. With a wide range of wind measurements and a high level of corrosion resistance, it is an excellent option. A reed switch is also present in this anemometer, which notes the times of rotation as the cups spin due to the velocity of the wind.cup Anemometer

Types of Anemometers

 

MUNRO manufacturesAnd markets a wide range of Portable Meteorological Sensors, Stationary Weather Sensors And Weather Stations
For various industries

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Information and Best Anaerobic Workstations /chambers

When working with materials that break down when exposed to oxygen, anaerobic workstations make sure there is no oxygen in the air.

Anaerobic workstations: These are stations that regulate the atmosphere. In a laboratory, when one works with components that break down when exposed to oxygen, anaerobic stations are made use.

Anaerobic jars: It is one of the instruments that is used in producing an environment that is anaerobic.

Comparison between the Anaerobic chamber and Anaerobic jars:

Anaerobic jars:

When a laboratory makes use of anaerobic jars, a completely anaerobic environment cannot be maintained. This is due to the possibility of leakages. Along with this, there is no chance of inspection of culture plates that are present in the anaerobic jars. This causes frequent disturbances in the anaerobic environment in the laboratory. Not only this, but it’s also costly to work with anaerobic jars in laboratories with a high anaerobic workload since they demand a lot of incubator area.

Anaerobic workstations:

These workstations prove to be more beneficial than the anaerobic jars as they provide better anaerobic conditions along with greater accuracy. These stations boost the rate of isolation of anaerobic pathogens, and they do not require disrupting the incubation. They can be examined as per demand without the anaerobic conditions being altered. They come at a much low expense than anaerobic jars too.

Ensure your workstation should have these:

 

  • The ease with the samples and operator entering and exiting the workstation is also a crucial point to note. This ensures that not too much disruption occurs to the anaerobic environment during entry and exit. After incubation, transferring of plates occurs. During this process, there is a possibility of oxygen exposure, so even this is to be kept in mind, and easy access to the workspace should be kept in mind.
  • Frequent monitoring of the workstation should be done. This includes checking of its temperature, anaerobic conditions, humidity, and all other factors.
  • As per the need of the laboratory, the architecture of the workstation fits your requirements accordingly. The size should be accurate, along with the amount of usable area it provides and the layout.

Use of anaerobic workstation’s chambers:

The anaerobic chamber was created to be used in microbiology. With changing times, it has changed to fulfil the demands of its operators and continues to be an important tool used in laboratories.  The anaerobic chamber is often used in investigations on organic matter, biofuels, biomass, and bioremediation, and also in the field of drug discovery and to identify contagious diseases.

 

Best anaerobic chambers

 

1.

Anaerobic Chamber/Workstation- 4 Gloves,800 Petri Dishes

The AW800TGRF4P is the largest workstation in the Electrotek range. One of the main advantages of the

unit is that despite its large capacity, it only occupies a modest amount of bench area. The workstation is

very economical to run, as two gas supplies are used.

Information and Best Anaerobic Workstations /chambers

2.

Anaerobic Chambers-2 Gloves,400 Petri Dishes

his range of workstations is ideal for the laboratory where oxygen free conditions are required for the

growth and identification of bacteria. The careful configuration gives the units a small bench area

yet ample working room within the incubator

Information and Best Anaerobic Workstations /chambers

3.

Anaerobic Workstation-2 Gloves,220 Petri Dishes

The AW200SG is the smallest of MUNRO’s range of anaerobic workstations. It is ideal for the laboratory

where oxygen free conditions are required for the growth and identification of bacteria.

Information and Best Anaerobic Workstations /chambers

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The Importance of Air Sampling

The Importance of Air Sampling:

 

Air sampling measures and monitors air quality to establish a healthy workplace. Depending on the company, it may be necessary to establish what particles are present in the air and how they may affect workers exposed to them.

What Does Air Sampling Involve?

Air sampling is a method for identifying airborne pollutants in a given environment. To detect air pollutants such as gases, vapors, dust, and fibers, specialized devices are employed. It is essential to collect air samples since inhaling these compounds can induce respiratory problems. Air sampling is frequently employed in firefighting operations, chemical production facilities, building sites, coal mining companies, and research laboratories to assess the quality of working life and protect people.

Consequently, Air Samplers enables businesses to evaluate the quality of the air and determine appropriate safety precautions. Air sampling is necessary for any industry in which airborne pollutants are widespread.

 Why Must Air Sampling Be Performed?

  • The safety of workers in the workplace could be determined too by checking the concentrations of the contaminants present in the air of the workplace.
  • The presence and level of harm of the pollutants can be identified by businesses through air sampling.
  • Air sampling can assist businesses and firms in making choices concerning air quality. Assessing the exposure of employees to airborne pollutants is a crucial aspect of the operations of most businesses.

The Importance of Air Sampling

Classifications of Air Sampling Tools:

Air Pollutants of various types include fumes, fibers, dust, mist, gases, vapors, etc. Various tools effectively capture carious pollutants.

  1. Inhalable Samplers: These samplers are designed to catch particles up to 100 micrometers in diameter. Companies use them to determine the risk of employee inhalation of contaminants.
  2. Filters: Porous membranes are used to pass air through the filter media. The air can travel through the small pores on the medium’s other side that traps pollutants. Substances such as PCTE, MCE, silver, quartz, PVC as well as glass fibre are employed in filter membranes to remove impurities from water and other liquids.
  3. Bubblers and Impingers: These are more effective on particles than bubblers are on gases and vapours. They gather impurities via the bubbling of air through a liquid binding the contaminants. They are frequently used in combination with an area pump to collect samples of pollutants in a specific region.
  4. Air sampling pumps: Airborne pollutants are gathered with the help of filter media and air sampling pumps. They are effective in air quality sampling done indoors. Among the pollutants, they operate effectively with hazardous particles, lead, asbestos, beryllium, mold spores, silica, and respirable dust.
  5. Cyclones: Filter cassettes are attached to cyclone assemblies to remove bigger, harmless particles from the air. Larger particles are pushed into grit pots by centrifuges that spin rapidly and require fast air rotation to do so. Filter cassettes then get clogged up with airborne particles.
  6. Filter cassettes: These cassettes use pumps to capture airborne particles. Mold samples are easily collected by such cassettes. Organizations can collect air pollutants from work settings for laboratory studies with their help of them too. To facilitate air sampling, cassettes are preinstalled with filters.

The Importance of Air Sampling

Varieties of Air Sampling:

  1. Personal: A worker (just one) has to normally wear testing equipment that is worn a few inches from his face. He is the only individual that can interact with the air. The apparatus collects a fair representation of the particles in interaction with the individual.

 

  1. Static: It is utilized to gather samples of ambient air. It is especially helpful for finding pollutants. Generally, sample devices for ambient air are typically bigger and also flow rates are high. It allows them to capture a large volume of air in a small span.

 

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A Guide to Microbiological Air Sampling

An air sampler comprises an inlet that directs air into the collector, a filter that removes bigger particles that could interfere with the analysis.

It is necessary to monitor medical and pharmaceutical plants with microbiological air monitoring.  It is considered a necessity in the majority of nations.  However, airborne germs and fungus may be just as dangerous in healthcare, food processing plants, office towers and other workplaces.  Large amounts of fungal spores in the air may have a detrimental influence on the shelf life of baked products.  It has its requirements in various other fields too.

methods for monitoring:

With the help of microbial air measurement, it is possible to determine the percentage of hyphae fragments and germinable spores present in the air.  Microbiological air aggregation devices that function in impaction are used to collect the samples.  Using a suction device, the microbiological air aggregation device draws in a specific volume of air. It then secretes the airborne spore via a sieve onto the underlying growth media in an agar plate.  It is recommended that the afflicted room not be ventilated for approximately 12 hours before the microbiological air assessment and that no dust is raised.

The two primary methods for monitoring are:

  1. Active sampling
  2. Passive monitoring

 

A Guide to Microbiological Air Sampling

Active Sampling of the Air

Active air sampling entails physically drawing a known air volume over a device of particle collection using a microbiological air sampler.  There are two primary varieties: impingers and impactors

 

Impingers

These capture particles using a liquid medium.  Usually, a sampled air is pulled into a tiny flask that contains collection medium via a suction pump with the help of a tube of narrow inflow.  This pushes the air toward the collection medium’s surface.  The rate of flow is governed by the entrance tube’s diameter.  The air quickly changes direction, impinging any suspended material into collection liquid whenever the airstrikes the liquid’s surface.  The collecting liquid could be cultivated to determine the presence of live bacteria after sampling.  The result is quantifiable, as the sample size can be determined with sampling and the flow rate.

Impingers have drawbacks for routine airborne microbiological testing.  Typically, traditional designs are built of glass, which is unsuitable for food and pharmaceutical manufacturing facilities.  Cells of some microbial organisms could be damaged as well as prolonged sample times may allow certain cells to grow inside the liquid collection media, which shouldn’t happen.

 Impactors

For gathering of particles, solid or adhesive media are used by impactors samplers. Owing to their convenience, they are also far more frequently employed in commercial uses than impingers.  Additionally, they are capable of handling greater flow rates and huge sample volumes required for monitoring air quality of clean rooms where microbes are anticipated to be negligible.  However, caution must be taken not to leave agar plates in the sampler heads for an extended period of time since the medium can dry out or even degrade.

A pump/fan draws air into the sampling head of a standard impactor sampler and accelerates it, typically by a sieve sampler, perforated plate or a slit sampler, narrow slit.  This results in laminar airflow onto the surface of collection, which is frequently a normal agar or contact plate loaded with an appropriate agar medium.  The size of the pores in sieve samplers, as well as the width of a slit in slit samplers, are used to calculate the air velocity.  When air collides with the collecting surface, it undergoes a change in direction that is tangential.  Inertia ejects any suspended particle colliding with the collection surface.  Once the proper air volume has been supplied through the sample device, the agar plate is okay to be withdrawn and immediately incubated.  Counting visible colonies after incubation provides a direct quantitative indication of the number of units that are colony-forming in the sampling air.

 

2.  Passive Monitoring:

Conventional Petri dishes with appropriate cultural medium that is unsealed and subjected for a specified period of time before being incubated for permitting visible colonies to grow and be counted.  Settle plates have a very restricted applicability as they’re only able to monitor living biological particles that settle outside the air then settle onto a surface with time.  They are also susceptible to contamination through sources that are non-airborne. They are unable to detect finer particles in air, and they are unable to sample exact amounts of air. Therefore, no quantitative results are found.  Settle plates are prone to be overrun in highly contaminated environments, and it can be challenging to interpret the data they generate.  If exposed for an extended period of time, the plates of the agar growth medium may degrade.  For the pros, they are advantageous for a qualitative investigation of airborne microorganisms and are affordable as well as simple to use.

 

MUNRO Air Samplers

They have a broad range of applications and are used widely in the nuclear, construction, agricultural and manufacturing industries.Air samples are taken to measure the concentration of harmful airborne contaminants. This is for health-and-safety purposes and to monitor compliance with industry regulations.

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Anaerobic Chamber’s Microbiological Significance

Anaerobic chambers are often called anaerobic glove boxes. These chambers are atmospheric control units for use while working with materials sensitive to oxygen or performing general isolation, etc. These machines simplify researchers to prepare, cultivate, and analyze samples without exposing them to ambient oxygen.

Anaerobic chambers have recently gained much importance in laboratories, especially for anaerobic processes and studies. Due to these anaerobic processes, there have been opportunities for new microbes to develop metabolically and physiologically using tools such as genomics, molecular, and proteomic tools. This has also widened the fields of applications of anaerobic chambers.

History of Anaerobic chambers

The invention of anaerobic chambers was done by Rolf Freter. He had been conducting studies on the anaerobic specimens that lived in the intestine of mouses. He took assistance from Dick Coy, an engineer in Michigan, to develop something through which he would have access to organisms. Because of this, he would have a supply of anaerobic organisms that is constant.

The anaerobic chamber that Dick Coy came up with was known to be the Coy Anaerobic Chamber. With time, as per the requirements of research, there were a lot of advancements in the technology of the anaerobic chambers. Among the very first used chambers were the vinyl chamber. Even after a long time, it is still the most extensively used. Later on, rigid chambers were designed for specialised purposes. Usually, the anaerobic environment with a concentration of 0–5 parts per million of oxygen is sustained using a mixture of hydrogen gas that reacts with a palladium catalyst, which eliminates the oxygen that is extra.

Anaerobic chamber applications:

Few of these applications include the usage of anaerobic chambers in clinical labs where for the patient specimens, the disease agents can be detected. We can also find if these diseases can be treated with the help of antibiotics through these chambers. These chambers can also help in the identification of the disease and studies on various topics such as the discovery of drugs, biofuels, bioremediation, biomass, etc.

Anaerobic Chamber’s Microbiological Significance:

Culture of microorganisms under anaerobic circumstances:

Anaerobic bacteria that are strict are extremely difficult to cultivate; they require specialised techniques right from collection to cultivation. Blood cultures often are collected and transported in separate vials: one including oxygen and one excluding. After that, they are cultured in the anaerobic chamber for research or phenotypic investigations. Incubation, which is a lengthy process, occurs in mediums of blood agar in an incubator at a temperature of around 37°C. Due to the chamber’s architecture, a direct investigation is permitted. Additionally, according to its size and qualities, the chamber can accept many samples. For instance, it can be used to create an environment that is suitable for tissues, microaerophilic organisms, or cells that are hypoxic. The anaerobic chamber is provided with sleeves to facilitate handling without interfering with anaerobic conditions.

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All you need to know about Anaerobic Chambers

Substances like metalloproteins are said to be oxygen sensitive. The reason behind this is that it is for the maintenance of a reduced state of the substance, an environment that does not have the presence of oxygen is needed. Substances like these could easily react with the oxygen in the environment as they are sensitive to it.

This makes the work done in anaerobic chambers to be a careful one. You need to have prior knowledge and skills before handling such anaerobic chambers.

As per the time taken, the experiments performed in aerobic chambers occupy less time than the anaerobic ones and make use of more effort and energy. You always have to keep in mind that the samples must not come in contact with an oxygenated environment. This is also one of the reasons why a lot of time is occupied.

Here is what you should know about anaerobic chambers prior to working with them:

  • Anaerobic chambers resemble big boxes of gloves. These could be either in sets of one or two depending on the number of times to be used.
  • If not like boxes, the anaerobic chambers resemble bags of polyvinyl that are flexible.
  • For the purpose of eradicating oxygen from the chamber always keep a catalyst of palladium in the chamber.
  • There is the presence of a transfer chamber that is airlock so that the chamber’s environment inside does not change due to bringing external substances inside it.
  • A mixture of 95% of Nitrogen along with 5% of Hydrogen is present in the chamber.

All you need to know about Anaerobic Chambers

Things to remember prior to working in the transfer chamber:

  • Degas all the samples from any oxygen. You can do this by bringing the items in the transfer chamber.
  • Keep a track of all the necessary materials that are required for the experiment. This avoids any contamination in the room and lowers the number of times you will have to go in and out of the chamber. Therefore keeping the gas safe from getting wasted.
  • All the necessary samples and other equipment for the experiment are supposed to be brought a night earlier in the chamber.
  • Renew the catalyst used in case the detector for oxygen detects it and goes off.
  • Argon/Nitrogen gas is an inert gas is capable of purging the solutions. Do it 30 to 60 minutes before taking the samples into the intake chamber. Purge or degas buffers/solutions for 30 minutes to an hour with an inert gas such as argon or nitrogen before you bring them into the intake chamber.
  • In the presence of the reductant, you are free to let the protein that is stable and requires disulfide reduction do the process of incubation without oxygen. Once the incubation process is done, inside the chamber you can do the overnight dialysis of the reductant.
  • If you find that there is not enough stability of the protein for dialysis then you can use inert gases to degas it. Before the process of disulfide reduction follow it by equilibrating inside the chamber.

All you need to know about Anaerobic Chambers

Remember these inside the anaerobic chamber:

  • Avoid bringing ice cubes inside as it leads to the presence of some amount of oxygen in the chamber. A Styrofoam box can be used instead by storing ice packs in it. Storing of samples can also be done by using nitrogen in liquid form, this freezes the samples. However, you need to take care of this:
  • There is a possibility of the pressure inside the chamber to increase as the liquid nitrogen is capable of releasing nitrogen gas and filling up the chamber. It is advisable to vacuum out half the gas in the liquid nitrogen. Be careful.
  • Do not leave behind any trash.
  • Items such as beakers, tips for pipets in all sizes, tabletop centrifuges, all kinds of tubes (microcentrifuge, conical), etc. should be stocked up in the chamber so that the next experimenter can make use of it.

Conclusion:

Not every chamber for the experiment is anaerobic. Other chambers can be found around the lab. However all of them are not anaerobic. The following should not be confused with anaerobic chambers: glove boxes of oxygen animal study and tissue culture, dry boxes, and full control of humidity.

If you’ve never come across Anaerobic Workstations, they can be quite daunting. Performing experiments involving gases, seals, redox states, vacuums, regulators, and precipitating or evaporating proteins can be difficult.  However, the anaerobic chambers can last for a long time efficiently and produce some interesting research if they are well-maintained.

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The Importance of Anti-Slip Pendulum Feet

It is of course true that there are a few things in life more important that the anti-slip feet on the bottom of a Pendulum.  You could even argue that there are many things of higher priority than the components of a BS 7976-2 Pendulum.  What should be of the utmost importance to any Pendulum operator however, is the integrity of the testing process and the accuracy of data produced, and the performance of anti-slip feet is supremely important in ensuring this.  This is why Munro Instruments are now offering upgraded Pendulum feet.

Practical Considerations

The basic principle of a Pendulum test is just that, a weighted pendulum.  The pendulum swings from a point of release, striking the floor and reading the slip resistance of that floor on the overswing.  If the pendulum doesn’t come in to contact with the floor then it swings freely to give a 0PTV result.

The pendulum Tester must swing freely in an arc about a fixed point.  Whilst the Pendulum frame is sturdy, it wouldn’t be practical to physically fix the machine to the floor, so movement of the frame across the floor has the potential to alter results produced.  This is of such importance that measures to increase stability of the frame were introduced by the UK Slip Resistance Group, namely a weighted back foot and anti-slip feet.

The anti-slip feet are a crucial part of a robust testing system.  With a weighted back foot and anti-slip feet, the frame is held securely in position, preventing movement and associated variability in results.

The Importance of Anti-Slip Pendulum Feet

UK Legal & Regulatory Requirements

Regardless of the practical benefits of testing with anti-slip feet, the feet are an essential component of a regulator-preferred and UKSRG-defined test process.  In short, if you fail to use anti-slip feet, you have not complied with the requirements of testing as directed by the HSE.

With slip resistance testing being so closely associated with slip and fall legal claims, it is essential that testing is conducted in accordance with industry best practice.  If your Pendulum doesn’t feature anti-slip pads for the feet, you cannot claim to have tested to the UK-preferred standard.   If you haven’t done the test correctly, you shouldn’t expect the results to hold any water in court.

Company No: 6965050  Registered Office:  44-45 Burnt Mill, Elizabeth Way, Harlow, Essex  CM20 2HU

If you are conducting tests outside the UK, I would strongly advise that you check your local standards/requirements as it is likely anti-slip feet have been incorporated as part of the test method given their previously discussed positive impact on accuracy.

The Munro Instruments Anti-slip Feet

The improved feet replace the existing levelling screws in the Pendulum frame.  Rather than the pointed tip which previously required an anti-slip pad to sit in, the new screws feature a sturdy plate with a rubberised bottom, essentially incorporating a small anti-slip pad in to the feet themselves.

Unlike other designs there is no movement between pad and foot which could lead to movement of the frame.  The rotational fixed pivot of the Munro design rotates with adjustment of the feet, but is otherwise solid and stable, maximising accuracy and repeatability.

The new feet are backwards compatible with all Munro Pendulums, and it is simply a case of swapping out your older levelling screws with the new ones provided.  A 5 minute job with a significant impact on both the accuracy and validity of results produced by your machine.

Having been subject to extensive testing in the field, the feet have proven to remain effective on a wide range of materials and in a wide range of conditions, and remain effective even on difficult surfaces such as slopes, stairs and aggressive profiles.

Benefits to Pendulum Operators

If, like me, you have been using a workable but ‘DIY’ pad system, it is well worth an upgrade.  Whilst my experience as a Pendulum operator is unusual, I conduct testing daily on sites across the UK, one benefit of the improved Munro anti-slip feet was immediately apparent to me.  With the feet attached to the Pendulum frame, I didn’t have to remove and set up the pads 36 times for every test, a blessing both for my aging frame and the efficiency of testing.

Further benefits of the new anti-slip feet include the protection of easily damaged surfaces from the harsh points of the old feet, and the knowledge that the essential pads weren’t going to be forgotten or misplaced during a test remit.

Crucially, the improved factory-fit feet provide excellent performance, and look as if they belong.  Aesthetics shouldn’t matter in testing, but any observer is likely to be given a greater sense of confidence in the results with a setup that both looks and performs professionally.

Order your anti-slip feet today – www.munro-instruments.com